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recombinant mouse gal 3  (R&D Systems)


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    Structured Review

    R&D Systems recombinant mouse gal 3
    Recombinant Mouse Gal 3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+gal+3/Recombinant+Mouse+Galectin-3+Protein/pm39483979-108-18-22
    Average 93 stars, based on 26 article reviews
    recombinant mouse gal 3 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: Altered galectin-3 distribution and migratory function in the pre-diabetic non-obese diabetic mouse thymus.
    Article Snippet: .. In those membranes treated only with BSA we added 2.5x106 thymocytes from both mice treated or not with recombinant mouse gal-3 (1197-GA, R&D Systems, Minneapolis, MN.) to the upper chamber (in 100 mL fugotaxis buffer containing 1% BSA in RPMI 1640 medium) and 600 mL of the same buffer in the lower chamber. ..

    Article Title: Delayed Galectin-3-Mediated Reprogramming of Microglia After Stroke is Protective.
    Article Snippet: Galectin-3 (Gal-3), a β-galactoside-binding lectin, has recently emerged as a molecule with immunoregulatory functions.. We investigated the effects of Gal-3 on microglia morphology, migration, and secretory profile under physiological conditions and in the context of ischemic injury.. We show that in the control conditions, exposure to recombinant Gal-3 increases microglial ramification and motility in vitro and in vivo via an IL-4-dependent mechanism.

    Article Title: Altered galectin-3 distribution and migratory function in the pre-diabetic non-obese diabetic mouse thymus
    Article Snippet: .. In those membranes treated only with BSA we added 2.5x10 6 thymocytes from both mice treated or not with recombinant mouse gal-3 (1197-GA, R&D Systems, Minneapolis, MN.) to the upper chamber (in 100 μL fugotaxis buffer containing 1% BSA in RPMI 1640 medium) and 600 μL of the same buffer in the lower chamber. ..

    Article Title: Thiamine pretreatment improves endotoxemia-related liver injury and cholestatic complications by regulating galactose metabolism and inhibiting macrophage activation.
    Article Snippet: Background: As a major metabolic site, the liver is an important target organ of endotoxemia.. High serum lipopolysaccharide (LPS) levels can cause hepatocyte necrosis and produce cholestasis, which results in severe liver injury.. Contrastingly, thiamine (THA) has shown anti-inflammatory effects against severe infections and may be indicated for systemic endotoxemia treatment.

    Article Title: Local administration of regulatory T cells promotes tissue healing.
    Article Snippet: .. Primary monocytes were isolated using the EasySep Mouse Monocyte Isolation Kit (STEMCELL Technologies), according to the manufacturer’s instructions, and cultured in DMEM/F12 (Thermo Fisher Scientific) with 10% FBS, 10 ng/ml M-CSF (PeproTech) and 100 units/ml penicillin/streptomycin, for 3 days, with or without 0.1 ng/ml IFN-γ (STEMCELL Technologies, #78021), and/or 1 ng/ml IL-1β (PeproTech, #211-11B), in the presence of one of the Treg-derived factors: recombinant mouse IL-10 (10 ng/ml, PeproTech, #210-10-10), recombinant human ANXA1 (500 ng/ml, R&D systems, #3770-AN-050), recombinant human ANXA2 (500ng/ml, R&D systems, #9409-AN-050), recombinant mouse FGL-2 (375 ng/ml, R&D systems, #5257-FL), recombinant mouse Areg (500ng/ml, PeproTech, #315-36), recombinant mouse Gal-1 (500 ng/ml, R&D Systems, #1245-GA), or recombinant mouse Gal-3 (500ng/ml, R&D Systems, #1197-GA). .. Monocytes were cultured at 37 °C with 5% CO2 for 3 days, while sorted Mo/MΦ were cultured for 48 h before detaching them with TrypLE (Thermo Fisher Scientific) containing 2mM EDTA and resuspending them in PBS for subsequent staining steps.

    Article Title: Upregulation of galectin-3 in immortalized Schwann cells IFRS1 under diabetic conditions.
    Article Snippet: A spontaneously immortalized adult Fischer rat Schwann cell line IFRS1 retains the characteristic features of normal Schwann cells, and can be a useful tool for the study of diabetic neuropathy.. In the present study, we examined the effects of high glucose and 3-deoxyglucosone (3-DG) on the viability and the protein expression of advanced glycation endproducts (AGE)-binding proteins, such as galectin-3 (GAL3) and receptor for AGE (RAGE) in IFRS1 cells.. Exposure to 30 mM of glucose or 0.2 mM of 3-DG for 7 days failed to impair the IFRS1 cell viability, but significantly upregulated the expression of GAL-3.

    Control:

    Article Title: Delayed Galectin-3-Mediated Reprogramming of Microglia After Stroke is Protective.
    Article Snippet: Galectin-3 (Gal-3), a β-galactoside-binding lectin, has recently emerged as a molecule with immunoregulatory functions.. We investigated the effects of Gal-3 on microglia morphology, migration, and secretory profile under physiological conditions and in the context of ischemic injury.. We show that in the control conditions, exposure to recombinant Gal-3 increases microglial ramification and motility in vitro and in vivo via an IL-4-dependent mechanism.

    Cell Culture:

    Article Title: Delayed Galectin-3-Mediated Reprogramming of Microglia After Stroke is Protective.
    Article Snippet: Galectin-3 (Gal-3), a β-galactoside-binding lectin, has recently emerged as a molecule with immunoregulatory functions.. We investigated the effects of Gal-3 on microglia morphology, migration, and secretory profile under physiological conditions and in the context of ischemic injury.. We show that in the control conditions, exposure to recombinant Gal-3 increases microglial ramification and motility in vitro and in vivo via an IL-4-dependent mechanism.

    Article Title: Local administration of regulatory T cells promotes tissue healing.
    Article Snippet: .. Primary monocytes were isolated using the EasySep Mouse Monocyte Isolation Kit (STEMCELL Technologies), according to the manufacturer’s instructions, and cultured in DMEM/F12 (Thermo Fisher Scientific) with 10% FBS, 10 ng/ml M-CSF (PeproTech) and 100 units/ml penicillin/streptomycin, for 3 days, with or without 0.1 ng/ml IFN-γ (STEMCELL Technologies, #78021), and/or 1 ng/ml IL-1β (PeproTech, #211-11B), in the presence of one of the Treg-derived factors: recombinant mouse IL-10 (10 ng/ml, PeproTech, #210-10-10), recombinant human ANXA1 (500 ng/ml, R&D systems, #3770-AN-050), recombinant human ANXA2 (500ng/ml, R&D systems, #9409-AN-050), recombinant mouse FGL-2 (375 ng/ml, R&D systems, #5257-FL), recombinant mouse Areg (500ng/ml, PeproTech, #315-36), recombinant mouse Gal-1 (500 ng/ml, R&D Systems, #1245-GA), or recombinant mouse Gal-3 (500ng/ml, R&D Systems, #1197-GA). .. Monocytes were cultured at 37 °C with 5% CO2 for 3 days, while sorted Mo/MΦ were cultured for 48 h before detaching them with TrypLE (Thermo Fisher Scientific) containing 2mM EDTA and resuspending them in PBS for subsequent staining steps.

    Isolation:

    Article Title: Local administration of regulatory T cells promotes tissue healing.
    Article Snippet: .. Primary monocytes were isolated using the EasySep Mouse Monocyte Isolation Kit (STEMCELL Technologies), according to the manufacturer’s instructions, and cultured in DMEM/F12 (Thermo Fisher Scientific) with 10% FBS, 10 ng/ml M-CSF (PeproTech) and 100 units/ml penicillin/streptomycin, for 3 days, with or without 0.1 ng/ml IFN-γ (STEMCELL Technologies, #78021), and/or 1 ng/ml IL-1β (PeproTech, #211-11B), in the presence of one of the Treg-derived factors: recombinant mouse IL-10 (10 ng/ml, PeproTech, #210-10-10), recombinant human ANXA1 (500 ng/ml, R&D systems, #3770-AN-050), recombinant human ANXA2 (500ng/ml, R&D systems, #9409-AN-050), recombinant mouse FGL-2 (375 ng/ml, R&D systems, #5257-FL), recombinant mouse Areg (500ng/ml, PeproTech, #315-36), recombinant mouse Gal-1 (500 ng/ml, R&D Systems, #1245-GA), or recombinant mouse Gal-3 (500ng/ml, R&D Systems, #1197-GA). .. Monocytes were cultured at 37 °C with 5% CO2 for 3 days, while sorted Mo/MΦ were cultured for 48 h before detaching them with TrypLE (Thermo Fisher Scientific) containing 2mM EDTA and resuspending them in PBS for subsequent staining steps.

    Incubation:

    Article Title: Upregulation of galectin-3 in immortalized Schwann cells IFRS1 under diabetic conditions.
    Article Snippet: A spontaneously immortalized adult Fischer rat Schwann cell line IFRS1 retains the characteristic features of normal Schwann cells, and can be a useful tool for the study of diabetic neuropathy.. In the present study, we examined the effects of high glucose and 3-deoxyglucosone (3-DG) on the viability and the protein expression of advanced glycation endproducts (AGE)-binding proteins, such as galectin-3 (GAL3) and receptor for AGE (RAGE) in IFRS1 cells.. Exposure to 30 mM of glucose or 0.2 mM of 3-DG for 7 days failed to impair the IFRS1 cell viability, but significantly upregulated the expression of GAL-3.

    Western Blot:

    Article Title: Upregulation of galectin-3 in immortalized Schwann cells IFRS1 under diabetic conditions.
    Article Snippet: A spontaneously immortalized adult Fischer rat Schwann cell line IFRS1 retains the characteristic features of normal Schwann cells, and can be a useful tool for the study of diabetic neuropathy.. In the present study, we examined the effects of high glucose and 3-deoxyglucosone (3-DG) on the viability and the protein expression of advanced glycation endproducts (AGE)-binding proteins, such as galectin-3 (GAL3) and receptor for AGE (RAGE) in IFRS1 cells.. Exposure to 30 mM of glucose or 0.2 mM of 3-DG for 7 days failed to impair the IFRS1 cell viability, but significantly upregulated the expression of GAL-3.

    other:

    Article Title: Local administration of regulatory T cells promotes tissue healing.
    Article Snippet: ELISAs for cytokines in injured muscle Homogenised muscle tissue was incubated for 30min on ice in T-PER Tissue Protein Extraction Reagent (Thermo Fisher Scientific) using 10ml/g of tissue containing 1 tablet of protease inhibitor (Roche)/7ml.



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